Introduction to the tutorial
Welcoming
This tutorial consists essentially of the above available Video. The use of mobile laboratories for legionella manual Measuring the concentration of legionella is explained.
In another section is the video of “technically supported” measurement for 40 to 100 samples to see.
For the successful measurement it is required, meticulously follow the steps shown. That is why it is recommended, several times to watch the video and to make personal notes.
Have fun seeing and much success in measuring!
Description and qualitative function:
- The living, active, effective Legionella be genetically "captured" and coupled to magnetic micro-bits. Legionella are separated by means of magnets, and rinses in several steps.
- By color tracer are made the bacteria visible.
- The number of Legionella is detected and then displayed photometrically.
- The sequence comprises a plurality of process steps. These steps take a total of about. 60 minutes. You can edit multiple samples simultaneously. While working full manual in Kit A, is offered in the kit B is a partially automated solution with a vacuum pump and an agitator.
Quantitative results:
- In analysis of a sample of 1000 ml is an accuracy of 60 … 106 KBE / CFU achieved per 1000ml.
- This corresponds to an accuracy of 6 CFU/100ml.
- Higher accuracies can be achieved by increasing the sample.
A publication in the newspaper of the Association of German pool professionals, in which the beneficial use of the Mobile Legionella Laboratory is described, is online here available.
Technically assisted measurement
If more than 3 Samples are to be analyzed simultaneously, then the kit B is recommended, Here are elements included, which facilitate the work with several samples.
Advantages and disadvantages
Hints:
- For each real measurement, a comparison measurement with a clean sample is always carried out. Therefore always be at least 2 sets consumed.
- The participation of the sampler in a training course in accordance with VDI 2047 or accreditation as a sampler for a laboratory is recommended but not required.
Applications:
- Review of water systems on infestation of legionella in drinking water, cooling water, water (industrial, public, private)
Disadvantage:
- This method is (still) not recognized by German authorities as a substitute of a laboratory measurement across cultures according to DIN EN ISO.
Advantages:
- Measurement results are immediately, after about. 60 minutes, available. The 10-day growing the cultures in an incubator is eliminated.
- The reaction rate for any exceedances and the leeway between institutional controls are considerably larger.
- The measurement can be made on site. As a result, distortions caused by transport, which is limited by standards at 24h, and a lack of cooling are impossible.
- Compared to the PCR method, the false positive results are avoided by already killed Legionella. Only living Legionella will be detected.
- Compared to the cultivation, better results are achieved, as well as so-called “not cultivable subspecies” Legionella are detected.
- The test can be carried out with its own trained personnel, that's why:
- Successful use on cruise ships of Carnival Cruise Lines.
- The American AOAC certification is available and has been validated by EMLabs in Philadelphia.
Kit A or Kit B
Mobile Legionella Laboratory Kit A for manual measurement:
- 1x storage and transport in plastic case,
- 1x photometric test device with Bluetooth USB dongle and activation code,
- 4x AAA batteries, 110V / 230V power supply with internal adapters, CD with software (Winows), printed manual in English,
- Small parts, of 4x 24mm / 10ml cuvette, 1x light protection cover, 1x stir bar, 1x dosing syringe 10ml, 1x cleaning brush, 1x cuvette holder, 1x magnetic unit for 4 cuvettes 10ml,
- Pay attention, no initial equipment with consumables or reagents included. Per measurement are 1 Filter Set and 1 Set reagents necessary.
Mobile Legionella Laboratory kit B for a filtration with a pump and an agitator for up 20 cuvettes:
- 1x storage and transport in aluminum case, Transport of the agitator outside the case,
- 1x photometric test device with Bluetooth USB dongle and activation code, 4x AAA batteries, 110V / 230V power supply with international adapters, CD with software (Windows), printed manual in English,
- 1x Adapter for 1ml Legionella test cuvettes
- Small parts, of 4x 24mm / 10ml cuvette, 1x light protection cover, 1x stir bar, 1x dosing syringe 10ml, 1x cleaning brush, 1x cuvette holder, 1x magnetic unit for 4 cuvettes 10ml
- 1x Agitator, electronic, with receiving means for 5 cuvettes, Cuvette holder magnet unit per 4 cuvettes 10ml, thats total of 20 cuvettes 10ml,
- 1x Table pad as a template made of woven plastic for correct storage of cuvette holder,
- 4x cuvette holder magnet unit for 4 cuvettes 10ml, Complement of hardware for the simultaneous processing of 19 + 1 samples,
- 16x 24mm / 10ml cuvette,
- 1x laboratory pipette (0,0 … 1,0ml),
- 10x elution cup 60ml,
- 1x filter equipment with 1x vacuum pump, 1x said filtration attachment 500ml, 1x glass bottle 1000ml, 1x tweezers, 1x scissors, 10x elution cup,
- consumables, included is initial equipment for until 99 measurements: 100x 47mm prefilter, 100x polycarbonate filter 0.40μm, 10x pipette tips 1ml,
- Pay attention, no initial equipment with reagents included. Per measurement are 1 Filter Set and 1 Set reagents necessary.
Upgrade from A to B
Upgrade Mobile Legionella Laboratory of Kit A Kit B on for a filtration with pump (Delivery is the difference between the A and B kit)
- 1x 1ml laboratory pipette (0.1 … 1.0 ml),
- 80x pipette tips for PLSP PIP 1ml laboratory pipette,
- 1x aluminum trolley with foam insert for accessories except MP4 agitator,
- 1x MP4 Multi-test agitator with 1x Electronic agitator with a special recording apparatus for inserting 5x MP4 rack with 20 Legionella cuvettes,
- 5x MP4-Rack, consisting of the cuvette holder for 4 cuvettes, the magnetic unit and the storage-foot,
- 1x Table backing of woven plastic for storage of the MP4 racks,
- 80x 60ml elution cups,
- 1x Professional filter equipment with 1x vacuum pump, 1x said filtration attachment 500 ml, 1x glass bottle 1000 ml, 1x tweezers, 1x scissors, 10x elution cup,
- 100x 47mm GF/C (Pre-) Filters for Professional Filter Equipment,
- 100x 47mm 0.40μm polycarbonate filters for professional filter equipment,
Consumption material
Legionella mobile laboratory in suitcase
Mobile Legionella Laboratory consumables kit A for manual filtering 1 times
- 1x 60ml elution vessel, 1x syringe with GF / C filters, 1x Micro-Filter,
- 1x 10ml syringe with luer-lock connector (2 units), 1x 50ml syringe with luer-lock connector, 1x stopper, 2x Stops filter end,
Mobile Legionella Lab consumables kit A for manual filtering 10 times
- 10x 60ml elution vessel, 1x syringe with GF / C filters, 1x Micro-Filter,
- 10x 10ml syringe with luer-lock connector (2 units), 1x 50ml syringe with luer-lock connector, 1x stopper, 2x Stops filter end,
Mobile Legionella Laboratory consumables kit A for manual filtering 40 times
- 40x 60ml elution vessel, 1x syringe with GF / C filters, 1x Micro-Filter,
- 40x 10ml syringe with luer-lock connector (2 units), 1x 50ml syringe with luer-lock connector, 1x stopper, 2x Stops filter end,
Mobile Legionella Laboratory consumables kit A for manual filtering 100 times
- 100x 60ml elution vessel, 1x syringe with GF / C filters, 1x Micro-Filter,
- 100x 10ml syringe with luer-lock connector (2 units), 1x 50ml syringe with luer-lock connector, 1x stopper, 2x Stops filter end,
Mobile Legionella Laboratory consumables kit B for filtering via pump 100 times, 50 … 99 measurements
- 80x pipette tips for PLSP-PIP1ml laboratory pipette, 80x 60ml elution cups, 100x 47mm GF / C pre-filters for professional filter equipment, 100x 47mm 0.40μm polycarbonate filters for professional filter equipment,
Reagents
Legionella mobile laboratory in suitcase
Mobile Legionella Laboratory reagent kit 10 for Kit A or Kit B
Suitable for at least 5 up to 9 tests , because 1 Zero test is required,
- 1x bottle 110ml with L0 reagent,
- 10x individual doses per 1ml with L1 reagent,
- 1x bottle 200ml with L2 reagent,
- 10x individual doses per 1ml with L3 reagent,
- 5x tetra-dose per 5ml with L4 reagent,
- 1x bottle 2ml with reagent L5,
- 10x CB 1ml cuvettes, 10x MHCB 9ml cuvettes, 5x disposable pipettes,
Mobile Legionella Laboratory reagent kit 40 for Kit A or Kit B
Suitable for at least 20 up to 39 tests , because 1 Zero test is required,
- 1x bottle 420ml with L0 reagent,
- 1x bottle 42ml with L1 with reagent,
- 1x bottle 750ml with L2 reagent,
- 1x bottle 42ml with L3 reagent,
- 15x tetra-dose per 5ml with L4 reagent,
- 1x bottle 4,4ml with reagent L5,
- 40x CB 1ml cuvettes, 40x MHCB 9ml cuvettes,
Mobile Legionella Laboratory reagent kit 100 for Kit A or Kit B
Suitable for at least 50 up to 99 tests , because 1 Zero test is required,
- 1x bottle 1050ml with L0 reagent,
- 1x bottle 105ml with L1 with reagent,
- 2x bottle 940ml with L2 reagent,
- 1x bottle 105ml with L3 reagent,
- 35x tetra-dose per 5ml with L4 reagent,
- 1x bottle 11ml bottle with reagent L5,
- 100x CB 1ml cuvettes, 100x MHCB 9ml cuvettes,
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